Alkyne group functional magnetic microparticles （PuriMag G- Alkyne Beads ）
- BrandPuriMag
- TypePuriMag G Series
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Ordering information |
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Name |
Cat. No. |
Vol. |
Scheme |
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G-Alkyne |
PMG034-2 |
2 ml |
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1. Overview
Click Chemistry describes rapid, selective "click" reactions between paired functional groups in mild aqueous solutions. This concept has evolved into a convenient, versatile, and reliable two-step coupling procedure for molecules A and B, widely adopted in biosciences, drug discovery, and materials science.
PuriMag? G-Alkyne, Alkyne-Functionalized Magnetic Nanoparticles are uniform, polymer-coated superparamagnetic nanoparticles. Their hydrophilic surface ensures excellent dispersibility, low non-specific adsorption, and easy handling in diverse buffers. The beads feature a high-density surface coating of alkyne functional groups, enabling covalent capture of functionalized proteins via Cu(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Workflow Requirements:
Target proteins must be metabolically, enzymatically, or chemically tagged with azide groups.
Post-coupling rigorous washing eliminates non-specifically bound proteins.
Unique surface chemistry ensures ultra-low background binding without surfactants.
Features & Advantages:
High binding capacity
Rapid, efficient coupling
Charge-neutral surface post-coupling
Stable covalent bonds with minimal ligand leakage
Low non-specific binding
Capacity: 1–20 mg protein or 0.1–2 mg peptide per gram beads
Caution: Alkyne beads are unstable in organic solvents and high urea concentrations.
Schematic Diagram of Coupling Mechanism:
2. product description
Product Specifications
Description
Polymer coated Fe3O4 nanoparticles
Particle Size
200 nm
Number of Beads
~1.7×1010 beads/mg
Matrix
Proprietary polymer
Functional group
Alkyne group
Group density
~100 μmole / g of Beads
Magnetization
60~70 EMU/g
Formulation
10mg/mL in DI water
Storage
1 year at 2~8 ℃. Do not freeze.
3. Instructions for Use
1.Add ~10 mg PuriMag? Alkyne Magnetic Nanoparticles to 25 mL deionized water.
2.After brief ultrasonication, add:
5 mg copper sulfate solution (5 mg in 0.5 mL H?O)
22 mg sodium ascorbate solution (22 mg in 1 mL H?O)
Stir mixture for 30 min.
3.Add azide-functionalized compound, stir overnight at RT.
4.Recover product via magnetic separation. Wash with:
Distilled water
PBS (pH 7.0)
5.Treat final product with EDTA to remove copper ions.
(For research use only!)
