■ 產(chǎn)品描述
PuriMag G-streptavidin鏈霉親和素磁珠通過鏈霉親和素和生物素之間的高親和力作用，可以綁定任何生物素標記的分子，如抗體、蛋白質(zhì)、多肽、DNA等。粒子具有較大的比表面積和很高的捕獲效率。PuriMag G-streptavidin-biotinbiomolecule復合物通過磁分離易于從溶液中分離，因此提供了一個快速、簡潔的磁性納米粒子標記的生物分子的方法。鏈霉親和素磁珠可用于各種下游分離過程：蛋白純化、細胞分離、免疫及分子檢測。鏈霉親和素-生物素（SA-Biotin） 系統(tǒng)具有極高的結(jié)合親和力（K =10^-15） ， 在生物領(lǐng)域具有廣泛的應用。本產(chǎn)品采用超順磁性微球，粒徑均一、比表面積大、形貌規(guī)整，有利于方便、快捷、高效地捕獲目標分子以及實現(xiàn)磁性分離。本產(chǎn)品可配套自動化設(shè)備進行高通量操作。
■ 產(chǎn)品信息
尺寸: 200 nm
濃度: 10 mg/ml
配基: Streptavidin 鏈霉親和素
磁性: 超順磁
包覆材料： GMA
磁分離時間<30S
儲存：Store at 2-8°C（up to 6 months）in Storage Buffer (25 mM tris-HCl, 0.15 M NaCl, 0.05% tween20, 0.09% NaN3, pH 7.2). Do not freeze the reagent.
■ 結(jié)合能力
>50 μg Streptavidin/mg beads;
>4000 pmol free biotin/mg beads;
>300 pmol biotin-oligonucleotide/mg beads;
>20 pmol biotin-dsDNA fragments/mg beads;
本產(chǎn)品可完全替代市場主流鏈霉親和素磁珠如GE Sera-Mag? Magnetic Beads Streptavidin、Pierce Streptavidin Magnetic Beads、Dynabeads? MyOne? 鏈酶親和素、JSR的Streptavidin MS150等產(chǎn)品，表現(xiàn)為等同甚至更優(yōu)的效果。
■質(zhì)量控制
結(jié)合能力：每批鏈霉親和素磁珠均用游離生物素測試保證的最小結(jié)合能力。
蛋白質(zhì)的非特異性結(jié)合：將2 mg SA磁性顆粒與0.2 mg IgG在200 μl的50 mM磷酸鹽緩沖液（0.15M NaCl，pH7.2）中溫育1小時（15-25℃）。用2×200 ul溫育緩沖液洗滌后，測上清液中的蛋白質(zhì)濃度（A280）。未檢測到非特定的結(jié)合。
無DNA酶活性：將1 μg的DNA底物于37℃下在含有1mg的 SA磁珠的100ml緩沖液M （10mM Tris-HCl，10mM MgCl2，50mM NaCl，1mM DTT，pH7.5）中溫育4小時。未檢測到DNase活性。
無RNA酶活性：將5ug MS2 RNA在37℃下在100ml 10mM Tris-HCl，pH7.5和1mg SA磁性顆粒中溫育4小時。未檢測到RNase活性。
無蛋白酶活性：將10mg的 SA磁珠與casein-resorufin在200μl的0.2M Tris-HCl（20mM CaCl2，pH 7.8）中于37℃溫育30分鐘。 TCA沉淀后，蛋白酶活性與上清液中574nm處的吸光度相關(guān)。沒有檢測到蛋白酶活性。
■ 如何使用鏈霉親和素磁珠
鏈霉親和素磁珠PuriMag G-streptavidin可以用于捕獲生物素biotin標記的抗體、蛋白質(zhì)、多肽、DNA等，可分為直接捕獲與間接捕獲兩種方式。如下所示：
■ 鏈霉親和素磁珠應用示例
例1. 生物素標記的核酸與SA磁珠的結(jié)合
例2. 鏈霉親和素磁珠固定化生物素化抗體用于化學發(fā)光
例3. 鏈霉親和素磁珠研究DNA/RNA與蛋白互作
鏈霉親和素磁珠詳細實驗方案見產(chǎn)品說明書，產(chǎn)品說明書請向客服索取。
■ 部分引用該磁珠文獻：
1. X. Li, C. Zhao, Y. Liu, Y. Li, F. Lian, D. Wang, Y. Zhang, J. Wang, X. Song, J. Li, Y. Yang, K. Xu, Fluorescence signal amplification assay for the detection of B. melitensis 16M, based on peptide-mediated magnetic separation technology and a AuNP-mediated bio-barcode assembled by quantum dot technology, Analyst (2019), 8 （鏈霉親和素磁珠PuriMag G-Strep偶聯(lián)生物素修飾多肽檢測致病菌B. melitensis 16M）
2. Yuxi Wei, Yue Wang, Jiawei Wang, Xiaolin Yang, Honglan Qi, Qiang Gao, Chengxiao Zhang . Homogeneous electrogenerated chemiluminescence immunoassay for the detection of biomarkers by magnetic preconcentration on a magnetic electrode, Analytical and Bioanalytical Chemistry，July 2019, Volume 411, Issue 18, pp 4203–4211（鏈霉親和素磁珠PuriMag G-Strep/ Homo-Strep偶聯(lián)生物素修飾抗體，免疫電化學熒光檢測生物標志物）
3. Chuchu Wang Xiaoyan Du Tiantian Xie Hao Li. Label‐ and modification‐free‐based in situ selection of bovine serum albumin specific aptamer, journal of separation Science. First published: 24 September 2019 （鏈霉親和素磁珠PuriMag G-Strep用于適配體篩選）
4. Hao Kong, Wei-wen Liu, Wei Zhang, Qiang Zhang, Cun-huai Wang, Muhammad Idrees Khan, Yu-xing Wang*, Liu-yin Fan*, and Cheng-xi Cao*. Facile, Rapid, and Low-Cost Electrophoresis Titration of Thrombin by Aptamer-Linked Magnetic Nanoparticles and a Redox Boundary Chip, ACS Appl. Mater. Interfaces, 2019, 11, 33, 29549–29556（鏈霉親和素磁珠PuriMag G-Strep用于DNA適配體偶聯(lián)）
5. Xiao-Li Chen, Hao Zheng, Wen-Ge Li, You-Hong Zhong, Xiao-Ping Chen, Jin-Xing Lu. Direct blood culturing of Candida spp. on solid medium was achieved by a rapid enrichment method with magnetic beads coated with rhMBL, Journal of Clinical Microbiology, 2020（鏈霉親和素磁珠PuriMag Homo-Strep 純化抗體）
6. Yang Feng , Neng-Bin Xie , Wan-Bing Tao , Jiang-Hui Ding , Xue-Jiao You , Cheng-Jie Ma , Xiaoxue Zhang , Chengqi Yi , Xiang Zhou , Bi-Feng Yuan , and Yu-Qi Feng. Transformation of 5-Carboxylcytosine to Cytosine Through C–C Bond Cleavage in Human Cells Constitutes a Novel Pathway for DNA Demethylation, CCS Chem. 2020, 2, 994–1008 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾DNA)
7. Haiping Wu, Min Zhao, Jia Li, Xiaoyan Zhou, Tiantian Yang, Dezhang Zhao, Ping Liu, Huangxian Ju, Wei Cheng*, and Shijia Ding*, Novel Protease-Free Long-Lasting Chemiluminescence System Based on the Dox-ABEI Chimeric Magnetic DNA Hydrogel for Ultrasensitive Immunoassay, ACS Applied Materials & Interfaces 2020, 12, 42, 47270-47277 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾DNA)
8. Luyao Shen, Keke Jia, Tao Bing*, Zhibao Zhang, Xiaoxiao Zhen, Xiangjun Liu, Nan Zhang, and Dihua Shangguan*. Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification, Anal. Chem. 2020, 92, 7, 5370–5378 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
9. Shanshan Yu, Limei Ai, Wei Wei, circRNA circ-MYBL2 is a novel tumor suppressor and potential biomarker in multiple myeloma, Human Cell, volume 34, pages219–228(2021) (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
10. Xi Rao Lingling Lai Xiaopeng Li Liang Wang Ai Li Qian Yang. N6‐methyladenosine modification of circular RNA circ‐ARL3 facilitates Hepatitis B virus‐associated hepatocellular carcinoma via sponging miR‐1305, IUBMB LIFE, Volume73, Issue2, February 2021, Pages 408-417(鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
11. Hongwei Hu , Yujing Ding , Zihan Gao , Hao Li. S1 nuclease digestion-based rational truncation of PD-L1 aptamer and establishment of a signal dual amplification aptasensor, Sensors and Actuators B Chemical, 2021, 331:129442, DOI:10.1016/j.snb.2021.129442(鏈霉親和素磁珠偶聯(lián)抑制因子篩選適配體)
12. Qing An, Zipeng Zhou, Yi Xie, Yu Sun, Haixiang Zhang & Yang Cao (2021) Knockdown of long non-coding RNA NEAT1 relieves the inflammatory response of spinal cord injury through targeting miR-211-5p/MAPK1 axis, Bioengineered, 12:1, 2702-2712, DOI:10.1080/21655979.2021.1930925 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
13. Dongyue Hu, Shusen Xiao, Qiaqia Guo, Rongrong Yue, Demin Geng and Debin Ji, Luminescence method for detection of aflatoxin B1 using ATP-releasing nucleotides, DOI: 10.1039/D1RA03870B (Paper) RSC Adv., 2021, 11, 24027-24031 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
14. Junhong Chen, Xiaoyan Zhuang, Jiyang Zheng, Ruofan Yang, Fei Wu, Aihui Zhang, Baishan Fang. Aptamer-based cell-free detection system to detect target protein, Synthetic and Systems Biotechnology, 2021, 6(3), 209-215, https://doi.org/10.1016/j.synbio.2021.07.004 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
15. Qiuyuan Lin, Zhipeng Huang, Xin Ye, Bin Yang, Xueen Fang, Baohong Liu, Hui Chen, Jilie Kong, Lab in a tube: Isolation, extraction, and isothermal amplification detection of exosomal long noncoding RNA of gastric cancer, Talanta, 225, 2021, 122090, https://doi.org/10.1016/j.talanta.2021.122090. ( 鏈霉親和素磁珠PuriMag G-Strep用于偶聯(lián)抗體捕獲外泌體)
16. Jinpeng Wu,Zengqi Tan,Hongjiao Li,Meixuan Lin,Yazhuo Jiang,Liang Liang,Qilong Ma,Junjie Gou,Lulu Ning,Xiang Li,Feng Guan,Melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O-GlcNAcylation of CDK5,Journal of Pineal Research, 2021,https://doi.org/10.1111/jpi.12765 (鏈霉親和素磁珠偶聯(lián)biotin糖蛋白)
17. Yuanbin Guo, Ming Shi, Xiujuan Liu, Huagang Liang, Liming Gao, Zhiwei Liu, Jian Li, Danjun Yu, Kun Li, Selection and preliminary application of DNA aptamer targeting A549 excreta in cell culture media, Microchemical Journal, Volume 171, 2021,106811,ISSN 0026-265X, https://doi.org/10.1016/j.microc.2021.106811.（鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體）
18. Gefei Li, Wenxiao Ma, Juan Mo, Boyang Cheng, Shin-ichiro Shoda, Demin Zhou*, and Xin-Shan Ye*，Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads，ACS Appl. Mater. Interfaces 2021, 13, 39, 46260–46269， https://doi.org/10.1021/acsami.1c11561 （鏈霉親和素磁珠偶聯(lián)寡糖）
19. Yaqi Ao, Anqi Duan, Binfen Chen, Xinmei Yu, Yaoyao Wu, Xiaojun Zhang, and Sanshu Li*. Integration of an Expression Platform in the SELEX Cycle to Select DNA Aptamer Binding to a Disease Biomarker, ACS Omega 2022, 7, 12, 10804–10811, https://doi.org/10.1021/acsomega.2c00769 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
20. Xue-Jiao You, Shan Zhang, Juan-Juan Chen, Feng Tang, Jingang He, Jie Wang, Chu-Bo Qi, Yu-Qi Feng, Bi-Feng Yuan, Formation and removal of 1,N6-dimethyladenosine in mammalian transfer RNA, Nucleic Acids Research, Volume 50, Issue 17, 23 September 2022, Pages 9858–9872, https://doi.org/10.1093/nar/gkac770 (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾RNA pull down)
21. Jinru Zhou, Yanbo Wang, Chaosheng Zhou, Lei Zheng, Linglin Fu, A ratiometric fluorescent aptasensor based on EXPAR to detect shellfish tropomyosin in food system, Food Control, Volume 144, 2023, 109380, https://doi.org/10.1016/j.foodcont.2022.109380. (鏈霉親和素磁珠PuriMag G-Strep用于biotin修飾適配體)
22. 丁于敬. 基于Real-SELEX的鹽酸沙拉沙星適配體篩選及其傳感器建立[D].北京化工大學,2021.DOI:10.26939/d.cnki.gbhgu.2021.000939. 23. 詹新宇. 基于DSN觸發(fā)RCA的功能化太赫茲超材料傳感平臺構(gòu)建及其在外泌體源性microRNA檢測中的應用[D].中國人民解放軍陸軍軍醫(yī)大學, 2022. DOI:10.27001/d.cnki.gtjyu.2022.000187. 24. 陽莎. 基于ID環(huán)3D編碼微球和THz技術(shù)的核酸類腫瘤標志物檢測平臺構(gòu)建及在結(jié)直腸癌中的應用[D].中國人民解放軍陸軍軍醫(yī)大學,2021.DOI:10.27001/d.cnki.gtjyu.2021.000060. 25. 孔昊. 基于適配體和電泳滴定的生物標志物檢測模型的開發(fā)和應用研究[D].上海交通大學,2019.DOI:10.27307/d.cnki.gsjtu.2019.003854. 26. 蔣靈麗. 抗羅紅霉素適配體的篩選和表征[D].華僑大學,2020.DOI:10.27155/d.cnki.ghqiu.2020.000439. 27. 凡蘇. 針對腫瘤標志物快速檢測的傳感器設(shè)計與應用研究[D].南京理工大學,2019.DOI:10.27241/d.cnki.gnjgu.2019.002203. 28. 李新新. 基于生物條形碼和量子點技術(shù)建立布魯氏菌16M的檢測方法[D].吉林大學,2019. 29. 郝江花. 基于靶點“鉤釣”策略的苦參堿抗PRRSV作用靶點的研究[D].山西農(nóng)業(yè)大學,2021.DOI:10.27285/d.cnki.gsxnu.2021.000460. 30. 曾靜怡. H1N1、H3N2、B型流感病毒適配體的篩選與表征[D].華僑大學,2021.DOI:10.27155/d.cnki.ghqiu.2021.000644. 31. 張浩哲. 基于血清輔助的PD-L1適配體篩選及魯棒性表征[D].北京化工大學,2022.DOI:10.26939/d.cnki.gbhgu.2022.001916. 32. 郭恒新. 基于環(huán)適體與滾環(huán)擴增的H1N1型流感病毒快速檢測方法的建立與應用[D].華僑大學,2021.DOI:10.27155/d.cnki.ghqiu.2021.000629. 33. 張智寶. 堿性磷酸酶異源二聚體的酶學性質(zhì)及其液體活檢中的應用研究[D].中國石油大學(北京),2020.DOI:10.27643/d.cnki.gsybu.2020.000283. 34. 王楚楚. 牛血清白蛋白適配體的無標記原位篩選及其傳感器的建立[D].北京化工大學,2019.DOI:10.26939/d.cnki.gbhgu.2019.000535. 35. 張偉. 水體中三種小分子環(huán)境污染物核酸適配體的制備及表征[D].華僑大學,2020.DOI:10.27155/d.cnki.ghqiu.2020.000003. 36. 張曉萌. 基于核酸適配體的生物毒素檢測方法建立及應用研究[D].昆明理工大學,2021.DOI:10.27200/d.cnki.gkmlu.2021.001730.
37. Jiankang Deng, et al. A novel ratiometric fluorescent aptasensor accurately detects patulin contamination in fruits and fruits products, Arabian Journal of Chemistry, Volume 15, Issue 2, 2022, 103569, https://doi.org/10.1016/j.arabjc.2021.103569. （SA磁珠偶聯(lián)適配體）
38. Guangyuan Wang, et al. Enzymatic Synthesis of DNA with an Expanded Genetic Alphabet Using Terminal Deoxynucleotidyl Transferase, ACS Synth. Biol. 2022, 11, 12, 4142–4155, https://doi.org/10.1021/acssynbio.2c00456 (SA磁珠偶聯(lián)核酸)
39. Zhimin Pan, et al.LncRNA TSIX knockdown restores spinal cord injury repair through miR-30a/SOCS3 axis, Biotechnology and Genetic Engineering Reviews, 2023, DOI: 10.1080/02648725.2023.2190948 （SA磁珠偶聯(lián)RNA）
40. He, Y., et al. Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk, Luminescence 2023, 38(5), 518. https://doi.org/10.1002/bio.4473 （SA磁珠偶聯(lián)適配體）
41. Ling, X., et al. Target Discovery of Matrine against PRRSV in Marc-145 Cells via Activity-Based Protein Profiling. Int. J. Mol. Sci. 2023, 24, 11526. https://doi.org/10.3390/ijms241411526 (SA磁珠偶聯(lián)小分子)
42. Ding, Jiang-Hui, et al. "Whole-genome map** of epigenetic modification of 5-formylcytosine at single-base resolution by chemical labeling enrichment and deamination sequencing." Analytical Chemistry 96.11 (2024): 4726-4735. （SA偶聯(lián)DNA）
43. He, Yugan, Yadan Peng, and Yigang Tong. "One-Tube Nested PCR Coupled with CRISPR-Cas12a for Ultrasensitive Nucleic Acid Testing." ACS Omega (2024).
44. Huang, Tianyu, et al. "In vitro selection and engineering azithromycin-specific aptamers and construction of a ratiometric fluorescent aptasensor for sensitive detection of azithromycin." Sensors and Actuators B: Chemical 411 (2024): 135789. （SA偶聯(lián)適配體）
45. Li X, Chen L, Liu T, et al. Integrated analysis of ATAC-seq and transcriptomic reveals the ScDof3-ScproC molecular module regulating the cold acclimation capacity of potato[J]. Plant Physiology and Biochemistry, 2024, 210: 108576.
46. Jiang Y, Wu J, Guan F, et al. O-GlcNAcylation determines the function of the key O-GalNAc glycosyltransferase C1GalT1 in bladder cancer: O-GlcNAcylation governs pro-tumorigenic role of C1GalT1 in bladder cancer[J]. Acta Biochimica et Biophysica Sinica, 2024, 56(8): 1108.
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