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Gefei Li, Wenxiao Ma, Juan Mo, Boyang Cheng, Shin-ichiro Shoda, Demin Zhou*, and Xin-Shan Ye*，Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads，ACS Appl. Mater. Interfaces 2021, 13, 39, 46260–46269，Publication Date:September 22, 2021
https://doi.org/10.1021/acsami.1c11561 （鏈霉親和素磁珠偶聯(lián)寡糖）

Abstract
Rapid diagnosis and vaccine development are critical to prevent the threat posed by viruses. However, rapid tests, such as colloidal gold assays, yield false-negative results due to the low quantities of viruses; moreover, conventional virus purification, including ultracentrifugation and nanofiltration, is multistep and time-consuming, which limits laboratory research and commercial development of viral vaccines. A rapid virus enrichment and purification technique will improve clinical diagnosis sensitivity and simplify vaccine production. Hence, we developed the surface-glycosylated microbeads (glycobeads) featuring chemically synthetic glycoclusters and reversible linkers to selectively capture the influenza virus. The surface plasmon resonance (SPR) evaluation indicated broad spectrum affinity of S-linked glycosides to various influenza viruses. The magnetic glycobeads were integrated into clinical rapid diagnosis, leading to a 30-fold lower limit of detection. Additionally, the captured viruses can be released under physiological conditions, delivering purified viruses with >50% recovery and without decreasing their native infectivity. Notably, this glycobead platform will facilitate the sensitive detection and continuous one-step purification of the target virus that contributes to future vaccine production.

摘要

快速診斷和疫苗開發(fā)對于預(yù)防病毒構(gòu)成的威脅至關(guān)重要。然而，快速檢測，如膠體金檢測，由于病毒數(shù)量少，會產(chǎn)生假陰性結(jié)果。此外，常規(guī)病毒純化，包括超速離心和納濾，是多步驟且耗時的，這限制了病毒疫苗的實驗室研究和商業(yè)開發(fā)?？焖俨《靖患图兓夹g(shù)將提高臨床診斷靈敏度并簡化疫苗生產(chǎn)。因此，我們開發(fā)了具有化學(xué)合成糖簇和可逆接頭的表面糖基化微珠（糖磁珠），以選擇性捕獲流感病毒。表面等離子共振 (SPR) 評估表明 S-連接糖苷對各種流感病毒具有廣譜親和力。磁性糖珠被整合到臨床快速診斷中，導(dǎo)致檢測下限降低 30 倍。此外，捕獲的病毒可以在生理條件下釋放，以 > 50% 的回收率提供純化的病毒，并且不會降低其天然感染性。值得注意的是，該糖珠平臺將有助于對有助于未來疫苗生產(chǎn)的目標(biāo)病毒進行靈敏檢測和連續(xù)一步純化。