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Luyao Shen, Keke Jia, Tao Bing*, Zhibao Zhang, Xiaoxiao Zhen, Xiangjun Liu, Nan Zhang, and Dihua Shangguan*. Detection of Circulating Tumor-Related Materials by Aptamer Capturing and Endogenous Enzyme-Signal Amplification, Anal. Chem. 2020, 92, 7, 5370–5378 (鏈霉親和素磁珠PuriMag G-Strep用于偶聯(lián)biotin修飾適配體)

原文請(qǐng)參考 https://pubs.acs.org/doi/abs/10.1021/acs.analchem.0c00051 

Abstract
Circulating tumor-related materials (CTRMs) shed from original or metastatic tumors, carry a lot of tumor information and are considered as important markers for cancer diagnosis and metastasis prognosis. Herein, we report a colorimetric detection strategy for CTRMs based on aptamer-based magnetic isolation and endogenous alkaline phosphatase (AP)-signal amplification. This strategy exhibited high sensitivity and selectivity toward the CTRMs that express AP heterodimers (the target of aptamer, a potential tumor marker). For clinical samples, this CTRM assay significantly discriminated colorectal cancer patients (n = 50) from healthy individuals (n = 39, p < 0.0001). The receiver operating characteristic (ROC) analysis indicated the sensitivity and specificity reached 92% and 82%, respectively, at the optimal cutoff point, the area under the curve of ROC reached 0.93, suggesting great potential for colorectal cancer diagnosis and therapeutic monitoring. Compared with CTC assays, this strategy is simple and has the potential for point-of-care testing.

從原始或轉(zhuǎn)移性腫瘤中脫落的循環(huán)腫瘤相關(guān)材料（CTRM），攜帶大量腫瘤信息，被認(rèn)為是癌癥診斷和轉(zhuǎn)移預(yù)后的重要標(biāo)志。在這里，我們報(bào)告基于適體的磁隔離和內(nèi)源性堿性磷酸酶（AP）信號(hào)放大基于CTRMs的比色檢測(cè)策略。該策略對(duì)表達(dá)AP異二聚體（適體的靶標(biāo)，一種潛在的腫瘤標(biāo)志物）的CTRM表現(xiàn)出高敏感性和選擇性。對(duì)于臨床樣品，該CTRM分析顯著區(qū)分了大腸癌患者（n = 50）和健康個(gè)體（n = 39，p <0.0001）。接受者操作特征（ROC）分析表明，在最佳截止點(diǎn)，ROC曲線下面積達(dá)到0.93，靈敏度和特異性分別達(dá)到92％和82％，這為大腸癌的診斷和治療監(jiān)測(cè)提供了巨大的潛力。與CTC分析相比，此策略簡(jiǎn)單易行，具有即時(shí)檢驗(yàn)的潛力。

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