客戶將氨基磁珠用于偶聯(lián)巰基PNA——論文通訊
2020-2-27 18:11:48點擊：
在本文中，我們報道了一種用于細(xì)胞角蛋白片段抗原21-1 DNA（CYFRA21-1 DNA）的超靈敏熒光檢測系統(tǒng)，用于肺癌的早期診斷。該方法使用以乙二胺四乙酸（EDTA）為金屬配體的電子轉(zhuǎn)移原子轉(zhuǎn)移自由基聚合（ARGET-ATRP）。首先，通過交聯(lián)劑將硫醇化肽核酸（PNA）與氨基磁珠（PuriMag G-NH2）連接，然后與CYFRA21-1 DNA（tDNA）雜交。隨后，通過與tDNA的磷酸基結(jié)合，將Zr4 +引入MBs，并通過磷酸-Zr4 +-羧酸酯化學(xué)方法將ARGET-ATRP的引發(fā)劑引入。接下來，將Cu（II）Br / EDTA通過抗壞血酸（AA）還原為Cu（I）/ EDTA，以觸發(fā)ARGET-ATRP，然后將大量的熒光素-o-丙烯酸酯（FA）分子從表面上接枝。 MB，可顯著放大熒光信號。在最佳條件下，tDNA在0.1 fM至1 nM（R2 = 0.9988）的范圍內(nèi)具有很強的線性關(guān)系。檢測限低至23.8 aM（?143個分子）?；贏RGET-ATRP策略的熒光檢測具有出色的靈敏度，選擇性，出色的抗干擾性能和成本效益。這些結(jié)果表明該策略在生物學(xué)檢測和早期臨床診斷中具有相當(dāng)大的潛力。
CuBr2/EDTA-mediated ATRP for ultrasensitive fluorescence detection of lung cancer DNA
J Zhang, Y Ba, Q Liu, L Zhao, D Wang, H Yang… - Journal of Advanced …, 2020 - Elsevier
… Experimental section. Materials and reagents. All chemicals and reagents were analytical grade,
and ultrapure water was used in all experiments. Aminated magnetic beads solutions (MBs, 10
mg mL ?1 ) were procured from PuriMag Biotech Co., Ltd. (Xiamen, China) …
and ultrapure water was used in all experiments. Aminated magnetic beads solutions (MBs, 10
mg mL ?1 ) were procured from PuriMag Biotech Co., Ltd. (Xiamen, China) …
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… Scheme. 1 Experimental Reagents and chemicals Aminated magnetic beads (MBs,
10 mg mL-1) solutions were purchased from PuriMag Biotech Co. Ltd. (Xiamen, China).
4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid …
10 mg mL-1) solutions were purchased from PuriMag Biotech Co. Ltd. (Xiamen, China).
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… Scientific Ltd. (Beijing, China). Magnetic nanoparticles (Fe 3 O 4 -MNPs, d = 200 nm,
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PuriMag Biotechnology Ltd. (Xiamen, China). Copper bromide …
10 mg mL ?1 ) modified with amino groups on the surface were purchased from
PuriMag Biotechnology Ltd. (Xiamen, China). Copper bromide …
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