文章快遞：客戶采用我司羧基磁珠偶聯(lián)噬菌體蛋白實現(xiàn)細(xì)菌捕獲和檢測
2025-5-3 12:50:44點擊：
Ding Y, Yang Q, Liu X, et al. An ultrasensitive fluorescence nano-biosensor based on RBP 41-quantum dot microspheres for rapid detection of Salmonella in the food matrices[J]. Food Chemistry, 2025, 468: 142504.
An ultrasensitive fluorescence nano-biosensor based on RBP 41-quantum dot microspheres for rapid detection of Salmonella in the food matrices
一種基于 RBP 41 量子點微球的超靈敏熒光納米生物傳感器，用于快速檢測食品基質(zhì)中的沙門氏菌
AbstractSwift screening of Salmonella-contaminated food is crucial for timely prevention and control of foodborne illness outbreaks. A novel phage receptor binding protein (RBP 41) was previously identified and characterized from phage T102. This study functionalized RBP 41 onto magnetic beads (MBs) and quantum dot microspheres (QDMs) to form magnetic separation and fluorescent probes, respectively. The bacteria were captured by RBP 41-MBs and labelled with RBP 41-QDMs to form MBs-RBP 41-bacteria-RBP 41-QDMs complexes, then the fluorescence intensity of complexes was detected for determination of Salmonella. This proposed biosensor was demonstrated to detect Salmonella as low as 0.1245 Log10 CFU/mL (～2 CFU/mL) within ～1.5 h. The recovery yield of Salmonella in the spiked food samples ranged from 87 % to 119 %, indicating that it could detect Salmonella in real samples. This novel magnetic fluorescence nano-biosensor has potential to detect the bacteria in the different sample to reduce the detection time and increase sensitivity.
迅速篩查受沙門氏菌污染的食物對于及時預(yù)防和控制食源性疾病暴發(fā)至關(guān)重要。先前從噬菌體 T102 中鑒定并表征了一種新的噬菌體受體結(jié)合蛋白 （RBP 41）。本研究將 RBP 41 功能化到磁珠 （MB） 和量子點微球 （QDM） 上，分別形成磁性分離和熒光探針。用 RBP 41-MBs 捕獲細(xì)菌，并用 RBP 41-QDMs 標(biāo)記，形成 MBs-RBP 41-bacteria-RBP 41-QDMs 復(fù)合物，然后檢測復(fù)合物的熒光強(qiáng)度，用于測定沙門氏菌。這種提出的生物傳感器被證明可在 ～1.5 小時內(nèi)檢測到低至 0.1245 Log10 CFU/mL （～2 CFU/mL） 的沙門氏菌。加標(biāo)食品樣品中沙門氏菌的回收率為 87% 至 119%，表明它可以在真實樣品中檢測到沙門氏菌。這種新型磁性熒光納米生物傳感器有可能檢測不同樣品中的細(xì)菌，以減少檢測時間并提高靈敏度。
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