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東南大學(xué)采用我司蛋白G磁珠偶聯(lián)抗體捕獲細(xì)菌進(jìn)行檢測(cè)發(fā)表論文

Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection

基于Ag陽(yáng)極溶解和陰極氧還原生物催化金黃色葡萄球菌檢測(cè)的時(shí)間傳感平臺(tái) 

An Ag@C hybrid bipolar electrode (BPE) sensing platform has been established for the temporal detection of Staphylococcus aureus (S. aureus) in food. Combining the advantages of anodic dissolution of Ag and cathodic biocatalysis of oxygen (O2) reduction, this strategy showed an ultralow detection limit down to 10 CFU mL?1. As the formation of Ag@C completely quenched the electrochemiluminescence (ECL) emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Meanwhile, S. aureus catalyzed the electrochemical reduction of O2 at the cathode, reducing the overpotential for cathodic O2 reduction and thus increasing the rate of anodic electron loss, facilitating Ag dissolution and restoring the ECL emission of luminol. When a constant potential was applied, through monitoring the ECL recovery time before and after the incubation of S. aureus on the cathode, S. aureus could be quantified due to the slight difference of the conductivity.

摘要 

建立了Ag@C混合雙極電極（BPE）傳感平臺(tái)，用于食品中金黃色葡萄球菌（S. aureus）的時(shí)間檢測(cè)。結(jié)合 Ag 的陽(yáng)極溶解和氧 (O2) 還原的陰極生物催化的優(yōu)勢(shì)，該策略顯示出低至 10 CFU mL-1 的檢測(cè)限。由于 Ag@C 的形成完全淬滅了魯米諾的電化學(xué)發(fā)光 (ECL) 發(fā)射，因此 ECL 發(fā)射恢復(fù)反映了陽(yáng)極溶解的程度。同時(shí)，金黃色葡萄球菌在陰極催化了O2的電化學(xué)還原，降低了陰極O2還原的過(guò)電位，從而增加了陽(yáng)極電子損失率，促進(jìn)了Ag的溶解，恢復(fù)了魯米諾的ECL發(fā)射。當(dāng)施加恒定電位時(shí)，通過(guò)監(jiān)測(cè)金黃色葡萄球菌在陰極上孵育前后的ECL恢復(fù)時(shí)間，由于電導(dǎo)率的微小差異，可以對(duì)金黃色葡萄球菌進(jìn)行量化。

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