客戶采用我司羧基聚合物磁珠偶聯(lián)抗體捕獲外泌體在《Talanta》發(fā)表論文
客戶采用我司4um羧基磁珠偶聯(lián)anti-CD63抗體，然后用于捕獲外泌體，裂解后反轉率等溫擴增，用于檢測。
Qiuyuan Lin, Zhipeng Huang, Xin Ye, Bin Yang, Xueen Fang, Baohong Liu, Hui Chen, Jilie Kong,
Lab in a tube: Isolation, extraction, and isothermal amplification detection of exosomal long noncoding RNA of gastric cancer,
Talanta,
Volume 225,
2021,
122090,
ISSN 0039-9140,
https://doi.org/10.1016/j.talanta.2021.122090.
(http://www.sciencedirect.com.group21-s.aronip.com/science/article/pii/S0039914021000114)
Abstract: Tumor-derived exosomes that inherit molecular information on parental cells hold great promise for cancer diagnostics. Currently, two main technical challenges, time-consuming and labor-intensive isolation of exosome and nucleic acid extraction with limited recovery that have restricted the detection of ultralow abundance exosomal nucleic acids. Here, we proposed a simple, efficient and “l(fā)ab in a tube” system for the detection of exosomal nucleic acids, which fully integrated exosomes enrichment using immunomagnetic beads (IMB) (10 min), fast exosomes lysis based on NP-40 lysate (5 min) and sensitive loop-mediated isothermal amplification (LAMP) in a tube. This method was demonstrated by detecting two exosomal long noncoding RNA biomarkers of gastric cancer (HOTTIP and lncRNA-GC1) with a dynamic detection ranging from 300 ng/μL to 10 ng/μL, and the detection limit of LAMP was 10 ng/μL. Additionally, this platform exhibited good performance in the analysis of exosomal HOTTIP RNA directly in human serum samples, which has the potential for detection of low-abundance exosomal nucleic acid biomarkers from cancers.
Keywords: Exosomal long noncoding RNA; Immunomagnetic beads isolation; Nucleic acid extraction; Loop-mediated isothermal amplification (LAMP)
摘要：腫瘤源性外顯子是遺傳親本細胞分子信息的重要來源，對腫瘤診斷具有重要的應用前景。目前，外顯子的耗時和勞動密集分離和回收率有限的核酸提取兩大技術難題限制了超低豐度外顯子核酸的檢測。本文提出了一種簡便、高效、“試管內實驗室”的體外核酸檢測系統(tǒng)，該系統(tǒng)利用免疫磁珠（IMB）（10min）、基于NP-40溶解酶（5min）和敏感環(huán)介導的等溫擴增（LAMP）技術，實現(xiàn)了完整的胞外體富集。本方法通過檢測胃癌（HOTTIP和lncRNA-GC1）兩個外顯體長非編碼RNA生物標記物，動態(tài)檢測范圍為300ng，證明了該方法的有效性/μL至10 ng/μ五十、 燈的檢測限為10ng/μL。該平臺在直接檢測人血清外周血中的外周熱尖RNA方面表現(xiàn)出良好的性能，有可能檢測腫瘤低豐度的外顯子核酸生物標志物。
關鍵詞：外顯子長非編碼RNA；免疫磁珠分離；核酸提?。画h(huán)介導等溫擴增（LAMP）
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