客戶采用我司氨基磁珠偶聯(lián)羧基化合物篩選適配體在《Sensors and Actuators B: Chemical》發(fā)表論文
客戶采用我司氨基磁珠（PuriMag G-NH2）偶聯(lián)羧基化合物篩選適配體。
Yujing Ding, Zihan Gao, Hao Li,
Real milk sample assisted selection of specific aptamer towards sarafloxacin and its application in establishment of an effective aptasensor,
Sensors and Actuators B: Chemical,
Volume 343,
2021,
130113,
ISSN 0925-4005,
https://doi.org/10.1016/j.snb.2021.130113.
(http://www.sciencedirect.com.group21-s.aronip.com/science/article/pii/S0925400521006821)
Abstract: The aptamers screened by the classic SELEX strategy or other approaches do not undergo the real sample analysis during the selection process, and the environmental inconsistency between the selection and final detection systems might influence the aptamer characteristics and detection performance. Herein, a novel real sample assisted SELEX selection strategy has been proposed to rationally select the suitable aptamers with high detection performance, using the selection of a sarafloxacin specific aptamer as an example. Based on the developed strategy, the aptamer Apt-F2 with higher specificity and SAR binding affinity was obtained as compared to the aptamer Apt-B4 selected by the standard SELEX strategy. Apt-F2 also exhibited a stronger conformational change after binding as compared to Apt-B4. Apt-B4 and Apt-F2 were used as a platform for developing the fluorescent aptasensor. Under optimized conditions, the limit of detection of Apt-B4- and Apt-F2-based aptasensors for SAR was 0.19 and 0.12?ng/mL respectively. Moreover, Apt-F2-based aptasensor also revealed a slightly superior SAR recovery rate from the spiked milk samples. The findings obtained in the study confirmed the advantage of the real SELEX strategy as compared to the traditional version. Further, the characteristics of the aptasensor proposed here also revealed its application potential for detecting SAR in the real milk or other animal-sourced food samples.
Keywords: Real SELEX strategy; Aptamer selection; Sarafloxacin; Milk; Aptasensor
摘要：通過經(jīng)典的SELEX策略或其他方法篩選出的適體在篩選過程中沒有經(jīng)過真實(shí)的樣本分析，而且篩選系統(tǒng)與最終檢測(cè)系統(tǒng)之間的環(huán)境不一致可能影響適體的特性和檢測(cè)性能。本文以薩拉氟沙星特異性核酸適體的選擇為例，提出了一種新的真實(shí)樣本輔助SELEX選擇策略，以合理選擇具有高檢測(cè)性能的適體。基于該策略，獲得了比標(biāo)準(zhǔn)SELEX策略選擇的Apt-B4具有更高特異性和SAR結(jié)合親和力的Apt-F2適體。與Apt-B4相比，Apt-F2在結(jié)合后表現(xiàn)出更強(qiáng)的構(gòu)象變化。以Apt-B4和Apt-F2為平臺(tái)，研制了熒光型aptasensor。在優(yōu)化的條件下，Apt-B4-和Apt-F2-基aptasensors對(duì)SAR的檢測(cè)限分別為0.19和0.12?ng/mL。此外，基于Apt-F2的aptasensor還顯示，從加標(biāo)牛奶樣品中獲得的SAR回收率略高。研究結(jié)果證實(shí)了真實(shí)SELEX策略相對(duì)于傳統(tǒng)策略的優(yōu)勢(shì)。此外，本文提出的aptasensor的特性也揭示了其在實(shí)際牛奶或其他動(dòng)物源食品樣品中檢測(cè)SAR的應(yīng)用潛力。
關(guān)鍵詞：實(shí)SELEX策略；適體選擇；沙拉沙星；牛奶；適體傳感器
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